Composite
Cu kill

Part:BBa_K5140001:Design

Designed by: Yuki Matsunaga   Group: iGEM24_TUPLS-Japan   (2024-10-02)


Copper ion-induced absolute bacterial kill switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 44
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 55
    Illegal NheI site found at 78
    Illegal SpeI site found at 44
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 44
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 44
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 523
    Illegal SapI.rc site found at 673


Design Notes

Nothing


Source

https://www.ncbi.nlm.nih.gov/gene/946106 https://www.ncbi.nlm.nih.gov/gene/55531637 https://www.ncbi.nlm.nih.gov/gene/945332/#reference-sequences

References