Composite
Cu kill
Part:BBa_K5140001:Design
Designed by: Yuki Matsunaga Group: iGEM24_TUPLS-Japan (2024-10-02)
Copper ion-induced absolute bacterial kill switch
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 44
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 55
Illegal NheI site found at 78
Illegal SpeI site found at 44 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 44
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 44
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 523
Illegal SapI.rc site found at 673
Design Notes
Nothing
Source
https://www.ncbi.nlm.nih.gov/gene/946106 https://www.ncbi.nlm.nih.gov/gene/55531637 https://www.ncbi.nlm.nih.gov/gene/945332/#reference-sequences